Proteinase K CAS 39450-01-6 inotengeswa zvakanyanya pasi rose ine mwero wepamusoro wekubuda pachena uye zuva razvino rekugadzirwa kwayo
Nhengo yega yega kubva kuboka redu guru remari inowana mari inokoshesa zvinodiwa nevatengi uye kutaurirana kwesangano kune vanotengesa zvakanyanya muFakitori. Yakakurumbira PasiroseProteinase K CAS 39450-01-6Nekukwira Kwehuwandu hwezvinhu zvinotengeswa uye Zuva Rekupedzisira reKugadzirwa, Parizvino, tiri kutarisira kushanda pamwe chete nevatengi vekune dzimwe nyika zvichibva pakubatsirana. Ita shuwa kuti haubhadhariswi kuti utibate kuti uwane rumwe ruzivo.
Nhengo yega yega kubva kuboka redu guru remari inowana mari inokoshesa zvinodiwa nevatengi uye kutaurirana kwesangano redu.China 39450-01-6, Tinogamuchira nemufaro rutsigiro rwenyu uye tichashandira vatengi vedu vari kumba nekune dzimwe nyika nezvinhu zvemhando yepamusoro uye sevhisi yakanaka yakanangana nekufambira mberi kwebhizinesi semazuva ese. Tinotenda kuti muchabatsirwa nehunyanzvi hwedu munguva pfupi iri kutevera.
| Mvura nhamba 1 | |
| Basa remapuroteni rakasiyana nere>800U/mL, huwandu hwezvinhu zviri muhuwandu i20mg/mL, hapana Nickase residue | |
| Nhamba 2 upfu | |
| Chitarisiko | Upfu hwakachena hwakasanganiswa |
| Kuchena kwemagetsi | ≥95% |
| Basa re enzyme | ≥30U/mg |
| Zvisikwa zveNucleic acid | Zvisingaonekwe |
| Zvisikwa zveDeoxyribonuclease | Zvisingaonekwe |
| Zvisikwa zveRibonuclease | Zvisingaonekwe |
| Nhamba yengoro | G1205-10ML |
| Kuchengetera | Kana ikachengetwa pa -20 °C, chigadzirwa ichi chinochengetedza basa kweinenge gore rimwe chete, kutakurwa kwebhegi rechando. |
Mapuroteni Ki serine protease iri mumhuri ye subtilisin ine enzyme activity inoshanda uye substrate specificity, iyo inonyanya kuputsa ester bonds nema peptides ari pedyo neC-terminus yema hydrophobic amino acids, sulfur-containing amino acids, uye aromatic amino acids bonds, ayo anowanzo shandiswa kuputsa mapuroteni kuti agadzire ma short peptides.Mapuroteni Kine hunhu hwechinowanzo shandiswa seAsp39-His69-Ser224, inongowanikwa chete ne serine proteases uye ine nzvimbo mbiri dzinosunga Ca2+ dzakatenderedza nzvimbo inoshanda kuti iwedzere kugadzikana kwayo uye kuchengetedza enzyme yakawanda pasi pemamiriro akasiyana-siyana.
1. Kiti yekuongorora majini.
2. Zvishandiso zvekubvisa RNA neDNA.
3. Bvisa zvinhu zvisiri zveprotein munyama uye bvisa tsvina yeprotein (yakadai sekugadzirira majekiseni eDNA neheparin).
4. Kugadzirira Chromosomal DNA yePulse Electrophoresis.
5. Kubvisa tsvina kubva kuWestern.
6. Kugadzirwa uye kugadzirwa kwe enzyme glycated albumin reagents mumunda we in vitro diagnostics.
1. 30mg/bhodhoro 1g/bhodhoro kana 100g/bhodhoro
2. 1kgs/bhegi refoiri
Proteinase K iprotease yakasimba ye serine ine hunhu hwakakura hwe substrate. Inoparadza mapuroteni akawanda ari munharaunda yacho kunyangwe paine sipo. Haishandiswe nesimbi ions, chelating agents (semuenzaniso, EDTA), sulfhydryl reagents, kana ne tripsin kana chymotrypsin inhibitors. Yakasimba pane pH yakakura (4–12.5), ine basa rakanaka pa pH 6.5–9.5. Basa rinogona kukurudzirwa nekuwedzera denaturing agents (SDS neUrea). Kukurumidza denaturation kwe enzyme kunoitika patembiricha iri pamusoro pe 70°C. Autolysis ye enzyme inoitika zvakanyanya pa alkaline pH. Zvisinei, Proteinase K haishande zvachose ne autolysis. Zvimwe zvikamu zve enzyme zvinoramba zvichichengetedza basa razvo re proteolytic, kunyangwe mushure me autolysis yakakura. Proteinase K inowanzoshandiswa mu molecular biology applications kugaya mapuroteni asingadiwe, akadai se nucleases muDNA kana RNA preparations kubva ku microorganisms, cultured cells, uye plants. Iyi enzyme inowanzo shandiswa pa50–200 ug/ml mukugadzirwa kwe nucleic acid pa pH 7.5–8.0 uye 37~55oC. Nguva yekufukidzwa kwembeu inosiyana kubva pamaminitsi makumi matatu kusvika maawa gumi nemasere.
Serine protease yakasimba uye inoshanda zvakanyanya; inoshandiswa pakupatsanura mapuroteni ne nucleic acid. Proteinase K inoshandiswa mukuchenesa RNA neDNA kubva mumatishu kana mitsetse yemasero.
Nhengo yega yega kubva kuboka redu guru remari inowana mari inokoshesa zvinodiwa nevatengi uye kutaurirana kwesangano kune Factory inotengeswa zvakanyanya pasi rose Proteinase K CAS39450-01-6Nekukwira Kwehuwandu hwezvinhu zvinotengeswa uye Zuva Rekupedzisira reKugadzirwa, Parizvino, tiri kutarisira kushanda pamwe chete nevatengi vekune dzimwe nyika zvichibva pakubatsirana. Ita shuwa kuti haubhadhariswi kuti utibate kuti uwane rumwe ruzivo.
Zvinotengeswa zvakanyanya mufekitoriChina 39450-01-6， Tinogamuchira rutsigiro rwenyu nemufaro uye tichashandira vatengi vedu vari kumba nekune dzimwe nyika nezvinhu zvemhando yepamusoro uye sevhisi yakanaka yakanangana nekuvandudzwa kwazvinoitwa nguva dzose. Tinotenda kuti muchabatsirwa nehunyanzvi hwedu munguva pfupi iri kutevera.
