mutengo wakaderera wefekitori Alkaline ProteinaseK CAS 39450-01-6
Takazvipira kupa mutengo unokwikwidzana, zvigadzirwa zvepamusoro uye mhinduro dzemhando yepamusoro, pamwe nekutumira nekukurumidza kune Alkaline yefekitori ine mutengo wakaderera.ProteinaseK CAS 39450-01-6, Tave tichida nemwoyo wese kushanda pamwe chete nevatengi pasi rese. Tinotenda kuti tichakugutsai. Tinogamuchirawo nemufaro vatengi kuti vauye kusangano redu vatenge zvigadzirwa zvedu.
Takazvipira kupa mutengo unokwikwidzana, zvigadzirwa zvepamusoro uye mhinduro dzemhando yepamusoro, pamwe nekutumira nekukurumidza kuneChina Alkaline ProteinaseK uye 39450-01-6, Nekuda kwemhando yepamusoro uye mitengo inonzwisisika, zvinhu zvedu zvakatumirwa kunyika dzinopfuura gumi nenzvimbo. Tiri kutarisira kushanda pamwe chete nevatengi vese vanobva kumba nekune dzimwe nyika. Uyezve, kugutsikana kwevatengi ndicho chinangwa chedu chekusingaperi.
| Mvura nhamba 1 | |
| Basa remapuroteni rakasiyana nere>800U/mL, huwandu hwezvinhu zviri muhuwandu i20mg/mL, hapana Nickase residue | |
| Nhamba 2 upfu | |
| Chitarisiko | Upfu hwakachena hwakasanganiswa |
| Kuchena kwemagetsi | ≥95% |
| Basa re enzyme | ≥30U/mg |
| Zvisikwa zveNucleic acid | Zvisingaonekwe |
| Zvisikwa zveDeoxyribonuclease | Zvisingaonekwe |
| Zvisikwa zveRibonuclease | Zvisingaonekwe |
| Nhamba yengoro | G1205-10ML |
| Kuchengetera | Kana ikachengetwa pa -20 °C, chigadzirwa ichi chinochengetedza basa kweinenge gore rimwe chete, kutakurwa kwebhegi rechando. |
Mapuroteni Ki serine protease iri mumhuri ye subtilisin ine enzyme activity inoshanda uye broad substrate specificity, iyo inonyanya kuputsa ester bonds nema peptides ari pedyo neC-terminus yema hydrophobic amino acids, sulfur-containing amino acids, uye aromatic amino acids bonds, ayo anowanzo shandiswa kuputsa mapuroteni kuti agadzire ma short peptides. Proteinase K ine hunhu hwe typical catalytic triad Asp39-His69-Ser224 yakasarudzika kune serine proteases uye ine nzvimbo mbiri dzeCa2+ dzinosunga dzakatenderedza active centre kuti iwedzere kugadzikana kwayo uye kuchengetedza high enzymatic activity pasi pemamiriro akasiyana-siyana.
1. Kiti yekuongorora majini.
2. Zvishandiso zvekubvisa RNA neDNA.
3. Bvisa zvinhu zvisiri zveprotein munyama uye bvisa tsvina yeprotein (yakadai sekugadzirira majekiseni eDNA neheparin).
4. Kugadzirira Chromosomal DNA yePulse Electrophoresis.
5. Kubvisa tsvina kubva kuWestern.
6. Kugadzirwa uye kugadzirwa kwe enzyme glycated albumin reagents mumunda we in vitro diagnostics.
1. 30mg/bhodhoro 1g/bhodhoro kana 100g/bhodhoro
2. 1kgs/bhegi refoiri
Proteinase K iprotease yakasimba ye serine ine hunhu hwakakura hwe substrate. Inoparadza mapuroteni akawanda ari munharaunda yacho kunyangwe paine sipo. Haishandiswe nesimbi ions, chelating agents (semuenzaniso, EDTA), sulfhydryl reagents, kana ne tripsin kana chymotrypsin inhibitors. Yakasimba pane pH yakakura (4–12.5), ine basa rakanaka pa pH 6.5–9.5. Basa rinogona kukurudzirwa nekuwedzera denaturing agents (SDS neUrea). Kukurumidza denaturation kwe enzyme kunoitika patembiricha iri pamusoro pe 70°C. Autolysis ye enzyme inoitika zvakanyanya pa alkaline pH. Zvisinei, Proteinase K haishande zvachose ne autolysis. Zvimwe zvikamu zve enzyme zvinoramba zvichichengetedza basa razvo re proteolytic, kunyangwe mushure me autolysis yakakura. Proteinase K inowanzoshandiswa mu molecular biology applications kugaya mapuroteni asingadiwe, akadai se nucleases muDNA kana RNA preparations kubva ku microorganisms, cultured cells, uye plants. Iyi enzyme inowanzo shandiswa pa50–200 ug/ml mukugadzirwa kwe nucleic acid pa pH 7.5–8.0 uye 37~55oC. Nguva yekufukidzwa kwembeu inosiyana kubva pamaminitsi makumi matatu kusvika maawa gumi nemasere.
Serine protease yakasimba uye inoshanda zvakanyanya; inoshandiswa pakupatsanura mapuroteni ne nucleic acid. Proteinase K inoshandiswa mukuchenesa RNA neDNA kubva mumatishu kana mitsetse yemasero.
Takazvipira kupa mutengo unokwikwidzana, zvigadzirwa zvepamusoro uye mhinduro dzemhando yepamusoro, pamwe nekutumira nekukurumidza kune Alkaline ProteinaseK yemufekitori nemutengo wakaderera.CAS39450-01-6, Tave tichida nemwoyo wese kushanda pamwe chete nevatengi pasi rese. Tinotenda kuti tichakugutsai. Tinogamuchirawo nemufaro vatengi kuti vauye kusangano redu vatenge zvigadzirwa zvedu.
mutengo wakaderera wefekitoriChina Alkaline ProteinaseK uye 39450-01-6, Nekuda kwemhando yepamusoro uye mitengo inonzwisisika, zvinhu zvedu zvakatumirwa kunyika dzinopfuura gumi nenzvimbo. Tiri kutarisira kushanda pamwe chete nevatengi vese vanobva kumba nekune dzimwe nyika. Uyezve, kugutsikana kwevatengi ndicho chinangwa chedu chekusingaperi.
