Mukurumbira wepamusoro Kutengesa Kunopisa Proteinase K CAS 39450-01-6 Hunhu Hwakavimbiswa
Tinofunga zvinofungwa nevatengi, kukurumidza kuita zvinhu zvinoenderana nezvinodiwa nevatengi, zvichibvumira kuti zvinhu zvive zvemhando yepamusoro, kuderedza mari yekugadzirisa, mari yekubhadhara inonzwisisika, zvakapa vatengi vatsva nevakare rutsigiro nerutsigiro rwekutengesa zvinhu zvine mukurumbira wakanaka.Proteinase K CAS 39450-01-6Hunhu Hwakavimbika, Senyanzvi mune izvi, takazvipira kugadzirisa chero dambudziko rekudzivirira kupisa kwakanyanya kune vashandisi.
Tinofunga zvinofungwa nevatengi, kukurumidza kuita zvinhu zvinoenderana nezvinodiwa nevatengi, zvichibvumira kuti zvinhu zvive nani, kuderedza mari yekugadzirisa, mari yekubhadhara inonzwisisika, zvakapa vatengi vatsva nevakare rutsigiro nerutsigiro.China Proteinase K neCAS 39450-01-6Kune michina yekugadzira nekugadzira yepamusoro uye vashandi vane hunyanzvi hwekuona kuti zvinhu zviri zvemhando yepamusoro. Tawana sevhisi yakanaka kwazvo isati yatengeswa, yatengeswa, uye yatengeswa kuitira kuti vatengi vagone kuodha. Kusvika pari zvino mhinduro dzedu dziri kufamba nekukurumidza uye dzakakurumbira muSouth America, East Asia, Middle East, Africa, nezvimwewo.
| Mvura nhamba 1 | |
| Basa remapuroteni rakasiyana nere>800U/mL, huwandu hwezvinhu zviri muhuwandu i20mg/mL, hapana Nickase residue | |
| Nhamba 2 upfu | |
| Chitarisiko | Upfu hwakachena hwakasanganiswa |
| Kuchena kwemagetsi | ≥95% |
| Basa re enzyme | ≥30U/mg |
| Zvisikwa zveNucleic acid | Zvisingaonekwe |
| Zvisikwa zveDeoxyribonuclease | Zvisingaonekwe |
| Zvisikwa zveRibonuclease | Zvisingaonekwe |
| Nhamba yengoro | G1205-10ML |
| Kuchengetera | Kana ikachengetwa pa -20 °C, chigadzirwa ichi chinochengetedza basa kweinenge gore rimwe chete, kutakurwa kwebhegi rechando. |
Mapuroteni Ki serine protease iri mumhuri ye subtilisin ine enzyme activity inoshanda uye substrate specificity, iyo inonyanya kuputsa ester bonds nema peptides ari pedyo neC-terminus yema hydrophobic amino acids, sulfur-containing amino acids, uye aromatic amino acids bonds, ayo anowanzo shandiswa kuputsa mapuroteni kuti agadzire ma short peptides.Mapuroteni Kine hunhu hwechinowanzo shandiswa seAsp39-His69-Ser224, inongowanikwa chete ne serine proteases uye ine nzvimbo mbiri dzinosunga Ca2+ dzakatenderedza nzvimbo inoshanda kuti iwedzere kugadzikana kwayo uye kuchengetedza enzyme yakawanda pasi pemamiriro akasiyana-siyana.
1. Kiti yekuongorora majini.
2. Zvishandiso zvekubvisa RNA neDNA.
3. Bvisa zvinhu zvisiri zveprotein munyama uye bvisa tsvina yeprotein (yakadai sekugadzirira majekiseni eDNA neheparin).
4. Kugadzirira Chromosomal DNA yePulse Electrophoresis.
5. Kubvisa tsvina kubva kuWestern.
6. Kugadzirwa uye kugadzirwa kwe enzyme glycated albumin reagents mumunda we in vitro diagnostics.
1. 30mg/bhodhoro 1g/bhodhoro kana 100g/bhodhoro
2. 1kgs/bhegi refoiri
Proteinase K iprotease yakasimba ye serine ine hunhu hwakakura hwe substrate. Inoparadza mapuroteni akawanda ari munharaunda yacho kunyangwe paine sipo. Haishandiswe nesimbi ions, chelating agents (semuenzaniso, EDTA), sulfhydryl reagents, kana ne tripsin kana chymotrypsin inhibitors. Yakasimba pane pH yakakura (4–12.5), ine basa rakanaka pa pH 6.5–9.5. Basa rinogona kukurudzirwa nekuwedzera denaturing agents (SDS neUrea). Kukurumidza denaturation kwe enzyme kunoitika patembiricha iri pamusoro pe 70°C. Autolysis ye enzyme inoitika zvakanyanya pa alkaline pH. Zvisinei, Proteinase K haishande zvachose ne autolysis. Zvimwe zvikamu zve enzyme zvinoramba zvichichengetedza basa razvo re proteolytic, kunyangwe mushure me autolysis yakakura. Proteinase K inowanzoshandiswa mu molecular biology applications kugaya mapuroteni asingadiwe, akadai se nucleases muDNA kana RNA preparations kubva ku microorganisms, cultured cells, uye plants. Iyi enzyme inowanzo shandiswa pa50–200 ug/ml mukugadzirwa kwe nucleic acid pa pH 7.5–8.0 uye 37~55oC. Nguva yekufukidzwa kwembeu inosiyana kubva pamaminitsi makumi matatu kusvika maawa gumi nemasere.
Serine protease yakasimba uye inoshanda zvakanyanya; inoshandiswa pakupatsanura mapuroteni ne nucleic acid. Proteinase K inoshandiswa mukuchenesa RNA neDNA kubva mumatishu kana mitsetse yemasero.
Tinofunga zvinofungwa nevatengi, kukurumidza kwekuita zvinhu zvinoenderana nezvinodiwa nevatengi, zvichibvumira kunaka kwemhando yepamusoro, kuderedza mari yekugadzirisa, mari yekubhadhara inonzwisisika, zvakapa vatengi vatsva nevakare rutsigiro nerutsigiro rweHigh Famous Hot Selling Proteinase K CAS.39450-01-6Hunhu Hwakavimbika, Senyanzvi mune izvi, takazvipira kugadzirisa chero dambudziko rekudzivirira kupisa kwakanyanya kune vashandisi.
Mukurumbira wepamusoroChina Proteinase K neCAS 39450-01-6Kune michina yekugadzira nekugadzira yepamusoro uye vashandi vane hunyanzvi hwekuona kuti zvinhu zviri zvemhando yepamusoro. Tawana sevhisi yakanaka kwazvo isati yatengeswa, yatengeswa, uye yatengeswa kuitira kuti vatengi vagone kuodha. Kusvika pari zvino mhinduro dzedu dziri kufamba nekukurumidza uye dzakakurumbira muSouth America, East Asia, Middle East, Africa, nezvimwewo.
