Dhizaini yeNyanzvi Yemhando Yepamusoro Proteinase K CAS Nhamba 39450-01-6 neMutengo Wakanakisisa
Tinowanzoita basa redu sevashandi vanobatika tichiva nechokwadi chekuti tinokupai zvinhu zvakanaka uye mutengo wepamusoro weProfessional Design High QualityMapuroteni K Nhamba yeCAS 39450-01-6neMutengo Wakanakisisa, Kuti uwane rumwe ruzivo uye chokwadi, ndapota taura nesu nekukurumidza sezvinobvira!
Tinowanzoita sevashandi vanobatika tichiva nechokwadi chekuti tinokupai zvinhu zvakanakisisa pamwe nemutengo wepamusoro wekutengesaChina Tritirachium Album Limber uye Molecular Biology, Kudzora kwakasimba kwemhando kunoitwa mune yega yega link yemaitiro ese ekugadzira. Tinovimba nemoyo wese kuti tichasimbisa kushandira pamwe kwakanaka uye kunobatsirana nemi. Zvichibva pamhinduro dzemhando yepamusoro uye sevhisi yakakwana yekutengesa usati watengesa/watengesa ndiyo pfungwa yedu, vamwe vatengi vakashanda nesu kwemakore anopfuura mashanu.
| Mvura nhamba 1 | |
| Basa remapuroteni rakasiyana nere>800U/mL, huwandu hwezvinhu zviri muhuwandu i20mg/mL, hapana Nickase residue | |
| Nhamba 2 upfu | |
| Chitarisiko | Upfu hwakachena hwakasanganiswa |
| Kuchena kwemagetsi | ≥95% |
| Basa re enzyme | ≥30U/mg |
| Zvisikwa zveNucleic acid | Zvisingaonekwe |
| Zvisikwa zveDeoxyribonuclease | Zvisingaonekwe |
| Zvisikwa zveRibonuclease | Zvisingaonekwe |
| Nhamba yengoro | G1205-10ML |
| Kuchengetera | Kana ikachengetwa pa -20 °C, chigadzirwa ichi chinochengetedza basa kweinenge gore rimwe chete, kutakurwa kwebhegi rechando. |
Mapuroteni Ki serine protease iri mumhuri ye subtilisin ine enzyme activity inoshanda uye substrate specificity, iyo inonyanya kuputsa ester bonds nema peptides ari pedyo neC-terminus yema hydrophobic amino acids, sulfur-containing amino acids, uye aromatic amino acids bonds, ayo anowanzo shandiswa kuputsa mapuroteni kuti agadzire ma short peptides.Mapuroteni Kine hunhu hwechinowanzo shandiswa seAsp39-His69-Ser224, inongowanikwa chete ne serine proteases uye ine nzvimbo mbiri dzinosunga Ca2+ dzakatenderedza nzvimbo inoshanda kuti iwedzere kugadzikana kwayo uye kuchengetedza enzyme yakawanda pasi pemamiriro akasiyana-siyana.
1. Kiti yekuongorora majini.
2. Zvishandiso zvekubvisa RNA neDNA.
3. Bvisa zvinhu zvisiri zveprotein munyama uye bvisa tsvina yeprotein (yakadai sekugadzirira majekiseni eDNA neheparin).
4. Kugadzirira Chromosomal DNA yePulse Electrophoresis.
5. Kubvisa tsvina kubva kuWestern.
6. Kugadzirwa uye kugadzirwa kwe enzyme glycated albumin reagents mumunda we in vitro diagnostics.
1. 30mg/bhodhoro 1g/bhodhoro kana 100g/bhodhoro
2. 1kgs/bhegi refoiri
Proteinase K iprotease yakasimba ye serine ine hunhu hwakakura hwe substrate. Inoparadza mapuroteni akawanda ari munharaunda yacho kunyangwe paine sipo. Haishandiswe nesimbi ions, chelating agents (semuenzaniso, EDTA), sulfhydryl reagents, kana ne tripsin kana chymotrypsin inhibitors. Yakasimba pane pH yakakura (4–12.5), ine basa rakanaka pa pH 6.5–9.5. Basa rinogona kukurudzirwa nekuwedzera denaturing agents (SDS neUrea). Kukurumidza denaturation kwe enzyme kunoitika patembiricha iri pamusoro pe 70°C. Autolysis ye enzyme inoitika zvakanyanya pa alkaline pH. Zvisinei, Proteinase K haishande zvachose ne autolysis. Zvimwe zvikamu zve enzyme zvinoramba zvichichengetedza basa razvo re proteolytic, kunyangwe mushure me autolysis yakakura. Proteinase K inowanzoshandiswa mu molecular biology applications kugaya mapuroteni asingadiwe, akadai se nucleases muDNA kana RNA preparations kubva ku microorganisms, cultured cells, uye plants. Iyi enzyme inowanzo shandiswa pa50–200 ug/ml mukugadzirwa kwe nucleic acid pa pH 7.5–8.0 uye 37~55oC. Nguva yekufukidzwa kwembeu inosiyana kubva pamaminitsi makumi matatu kusvika maawa gumi nemasere.
Serine protease yakasimba uye inoshanda zvakanyanya; inoshandiswa pakupatsanura mapuroteni ne nucleic acid. Proteinase K inoshandiswa mukuchenesa RNA neDNA kubva mumatishu kana mitsetse yemasero.
Tinowanzoita basa redu sevashandi vanobatika tichiva nechokwadi chekuti tinokupai zvakanakisisa uye mutengo wepamusoro weProfessional Design High Quality Proteinase K CAS No. 39450-01-6 nemutengo wakanakisisa, Kuti uwane rumwe ruzivo neruzivo, ndapota taurai nesu nekukurumidza sezvinobvira!
Dhizaini yeUnyanzviChina Tritirachium Album Limber uye Molecular Biology, Kudzora kwakasimba kwemhando kunoitwa mune yega yega link yemaitiro ese ekugadzira. Tinovimba nemoyo wese kuti tichasimbisa kushandira pamwe kwakanaka uye kunobatsirana nemi. Zvichibva pamhinduro dzemhando yepamusoro uye sevhisi yakakwana yekutengesa usati watengesa/watengesa ndiyo pfungwa yedu, vamwe vatengi vakashanda nesu kwemakore anopfuura mashanu.
